Jimmy Fallon took aim at Donald Trump’s marriage Monday evening, joking that the president’s wife, Melania Trump, was set to run off with one of his political allies amid her notable absence from the spotlight.

“Over the weekend, President Trump made a campaign stop in Long Island and Melania Trump did not travel with him,” Fallon started off during his Monday monologue for “The Tonight Show.” “Yep, people have noticed that she’s been laying low. In fact, she hasn’t been seen in public in 29 days.”

He added: “Trump heard and was like, ‘Big deal. I haven’t seen her in private for two years.”

This joke garnered a big response from the “Tonight Show” studio audience, who burst out laughing and cheered at the jab about the Trumps’ relationship. Fallon didn’t stop there, however.

“I understand Melania’s taken a step back, with everything going on, she’s worried at any moment she could be trapped with Trump inside a catering truck,” Fallon said, referencing the time the president hid in an airport catering truck to switch planes under the radar while facing an assassination threat from Iran.

Fallon concluded his commentary about the Trumps’ marriage with one last joke, telling the crowd: “Yep, no one has seen Melania. All I know is that it’s going to be crazy when we find out she ran off with Mitch McConnell.”

This remark appeared to take Fallon’s audience by surprise, as the crowd audibly responded within seconds. While some could be heard hooting and cheering in response, others appeared to let out a low groan of sorts.

Watch the moment below. A representative for the White House did not immediately respond to TheWrap’s request for comment.

Of course, Fallon’s remark was merely a joke, calling back to Sen. McConnell (R-Ky.)’s own lengthy absence this summer. As we previously reported, questions arose regarding McConnell’s well-being after the senator was hospitalized in mid-June and his office gave few updates.

He later released a photo and statement in mid-July, sharing he was hospitalized after becoming “briefly unconscious” following “a fall.”

“My doctors have confirmed that I didn’t break any bones or suffer a concussion. I didn’t have a heart attack or a stroke. I don’t have any tumors or hemorrhages,” he shared at the time. “But I was briefly unconscious and was taken to the hospital.”

He added: “And on the advice of my doctors, I won’t be able to return to the Senate floor to vote quite yet. But rest assured that, in the meantime, I’m not taking a break from the Senate business that matters to you. I’ve been working closely with my legislative staff on current issues, and with my Kentucky team who help me provide timely constituent services across our Commonwealth.”

Though McConnell has since been discharged from a rehabilitation center, he’s yet to return to Washington, D.C.

“The Tonight Show Starring Jimmy Fallon” airs weeknights at 11:35 p.m. ET on NBC.

Rosie O’Donnell stepped in as host for Jimmy Kimmel Monday evening and made herself right at home by taking aim at President Donald Trump during her opening monologue.

“They said you can’t come home again, but here I am,” O’Donnell, who received a standing ovation at the start of the show, said at the beginning of her monologue. “And if you thought the president hated ‘Jimmy Kimmel Live’ when it was hosted by Jimmy Kimmel, buckle up, people, buckle up.”

Later on in her monologue, O’Donnell reflected on her decision to leave the United States for Ireland in 2025, which came about after longtime nemesis Trump started his second term as president.

“I’ve been living in Ireland for the past two years, because of Mango Mussolini, I read that Project 2025 and said, ‘Got to get myself out of here,’” she recalled. “And I decided to go to Ireland, a place where people are driven to drink, regardless of who’s running their country.”

O’Donnell then recalled how her child responded to learning of their move, telling her studio audience, “They went to school and proceeded to tell their entire class, ‘I’m very sorry. I won’t be able to graduate sixth grade with you. I’m being forced to move to another country because the president of the United States hates my mother. But in all fairness, I believe my mother hates him more.’”

The comedian then took a moment to call out President Trump directly, noting into the camera, “Hi, I know you’re watching.”

She poked fun at Trump one last time before signing off her monologue, telling the audience, “And you know, right now he’s in the White House crying ’cause I haven’t mentioned him.”

O’Donnell notably did not say Trump’s name once during her monologue.

Watch her full monologue below.

O’Donnell’s appearance on “Jimmy Kimmel Live” came two months after the eponymous host confirmed that she would be one of his guest hosts during his summer break.

“Well, I hope you’re paying attention this summer because I will be taking the next two months off,” Kimmel told his right-hand Guillermo Rodriguez in June. “This time voluntarily.”

He continued: “But we have assembled a potent group of hosts to fill in for me, beginning with Tiffany Haddish, Colman Domingo, Ike Barinholtz, Anthony Anderson, Jelly Roll and, as a special treat for our commander-in-chief, I asked one of his all-time favorites, Rosie O’Donnell, to be here to keep the hits coming.”

O’Donnell was a notable choice at the time, given her nearly two-decade feud with Trump – which has seen the actress and the president exchange a number of jabs at one another over the years

In response to Kimmel’s invitation, O’Donnell took to Instagram and wrote, “And I can’t wait!!!”

“Jimmy Kimmel Live!” airs weeknights at 11:35 p.m. ET on ABC.